Lazar D. Nesovic

4 Questions 8 Answers 0 Followers

Questions related from Lazar D. Nesovic

Can different types of murine T-regs be stimulated and intracellularly stained as a one whole cell population?

I want to analyze the following T regulatory cell phenotypes: CD4+CD25+IL-10+ T regs, CD4+CD25+Foxp3+ T regs, and CD4+LAP+ (TGF-beta1-secreting) T regs. The IL-10+ and LAP+ T regs seem to have to...

20 July 2023 981 0 View

Is a 1:12.5 dilution of a CD45 antibody too high for staining murine skin leukocytes?

I am staining murine skin cells with a variety of fluorophores. When staining with CD45, I only start observing clear separation of positive/negative populations at a 1:12.5 dilution ( 8...

30 August 2021 8,876 7 View

Recommendations on a drawing software to represent an immunological process in a human body?

I am looking for an efficient drawing software to represent certain immunological processes for a scientific publication. Any recommendations are appreciated.

04 April 2020 8,254 0 View

Fluorescent labeling of a large concentration of Ovalbumin?

I need to fluorescently label 40 mg/ml of chicken ovalbumin. Most labeling kits require the concentration of protein solution not to exceed 2 mg/ml. Is there an efficient way/protocol to label...

29 May 2019 7,152 0 View