Lazar D. Nesovic

2 Questions 6 Answers 0 Followers

Questions related from Lazar D. Nesovic

Can different types of murine T-regs be stimulated and intracellularly stained as a one whole cell population?

I want to analyze the following T regulatory cell phenotypes: CD4+CD25+IL-10+ T regs, CD4+CD25+Foxp3+ T regs, and CD4+LAP+ (TGF-beta1-secreting) T regs. The IL-10+ and LAP+ T regs seem to have to...

20 July 2023 908 0 View

Is a 1:12.5 dilution of a CD45 antibody too high for staining murine skin leukocytes?

I am staining murine skin cells with a variety of fluorophores. When staining with CD45, I only start observing clear separation of positive/negative populations at a 1:12.5 dilution ( 8...

30 August 2021 8,834 7 View