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Questions related from Lazar D. Nesovic
I want to analyze the following T regulatory cell phenotypes: CD4+CD25+IL-10+ T regs, CD4+CD25+Foxp3+ T regs, and CD4+LAP+ (TGF-beta1-secreting) T regs. The IL-10+ and LAP+ T regs seem to have to...
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I am staining murine skin cells with a variety of fluorophores. When staining with CD45, I only start observing clear separation of positive/negative populations at a 1:12.5 dilution ( 8...
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I am looking for an efficient drawing software to represent certain immunological processes for a scientific publication. Any recommendations are appreciated.
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I need to fluorescently label 40 mg/ml of chicken ovalbumin. Most labeling kits require the concentration of protein solution not to exceed 2 mg/ml. Is there an efficient way/protocol to label...
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