Lax Lee

6 Questions 2 Answers 0 Followers

Questions related from Lax Lee

Human BCL2 protein with N-6x His tag purification- expression and purification help?

Hello, I have been trying to express and purify full length human BCL-2 protein in E coli. BCL-2 cDNA determined to be correct were introduced into Gtaeway pDEST 17 vector to allow for the...

14 July 2016 5,945 4 View

Cell seeding in 96 well and 6 well plate for cell line and protein assays?

I did my cell line assay in 96 well plate and wanted to do the corresponding protein assay in 6 well plate. For 96 well plate the seeding density i used was 40,000cells / well in 100ul final...

22 April 2016 5,916 1 View

How much concentration of calcium to add in cell line???

For one of my cell culture assays, i need to add additional calcium. The culture medium i use has 1.6mM calcium. Do I need to count this. I am interested i effective concentration suitable for...

12 April 2016 8,444 1 View

Cell line calculation??

I have used 40,000 cells/well for my cell line cytotoxicity assay in 96 well plates. the volume was 60ul. i have used 10ul of 100uM concentration of drug. Now I want to do immunoblotting of the...

21 March 2016 5,335 2 View

Need to conform the interaction of protein A and B?

Hypothetically i need to look at whether my protein say A in the presence of a drug destroys protein B in cell lines. I have in vitro cell lines assays to show this but I need to substantiate...

13 January 2016 2,058 2 View

Human Lipid anchor, membrane protein with c terminal GST tag...no overexpression of recombinat protein. Do you have any suggestions?

I have been trying to over express a human protein (membrane, lipid anchor), 75kDa size with a c terminal GST tag in E.coli. How ever i am not getting my protein of interest in coomassie or WB. I...

21 September 2015 2,275 6 View