Do I need to add an additional 'G' before my 20bp gRNA sequence during cloning (under U6) if the first nucleotide of my gRNA is already a 'G'?

Hello, according to published papers: https://www.nature.com/articles/nprot.2013.143/figures/4 It is recommended to have an extra guanine before the 20 bp gRNA sequence, for my efficient...

18 October 2024 9,714 1 View