Why do I get a low concentration of DNA after plasmid extraction (GigaPrep MN kit) ?

I've transformed high-copy plasmid (pGEMT Easy - 13 Kbp) in E.coli (JM109) and did plasmid extraction with commercial kit from Macherey Nagel GigaPrep EF. However, the resulted concentration of...

11 November 2019 3,099 8 View