Lauri Nikkanen

4 Questions 7 Answers 0 Followers

Questions related from Lauri Nikkanen

Effect of cyclic electron transfer via the NDH complex on the plant chloroplast ATP synthase conductivity parameter (gH+) ?

The conductivity of the chloroplast ATP synthase is usually estimated from the time constant of a first-order exponential fit to the post-illumination relaxation kinetics of the electrochromic...

01 November 2017 5,725 1 View

Determining the redox state of the PQ pool in dark from the OJIP transient of chlorophyll fluorescence?

I'm trying to use the fluorescence level at the J phase of the OJIP transient to roughly determine the redox state of the plastoquinone pool in darkness in Arabidopsis leaves. I compare the values...

31 January 2017 5,428 12 View

Does adding 5 mM DTT quench AMS alkylation of thiols in a protein sample?

I am trying to study the in vivo redox state of certain proteins in plant chloroplasts by alkylating free thiols in a TCA-precipitated total extract by AMS. Previously I have used PEG-MAL...

19 October 2015 1,203 3 View

Aggregation problem with MAL-PEG thiol-labelling of plant proteins and SDS-PAGE

I am trying to determine the in vivo redox states of several stromal proteins in Arabidopsis chloroplasts by labelling them with MAL-PEG and then running on an SDS-PAGE. MAL-PEG binds to reduced...

22 August 2014 4,193 9 View