Laurent Manchon

7 Questions 4 Answers 0 Followers

Questions related from Laurent Manchon

Is it correct to average LOD ?

--Hi, I've calculated several LODs in qPCR (one LOD per probe) and I'd like to know if it's okay to average the LODs for my experiment? thank you --

11 December 2024 8,605 0 View

Compute pvalue from ddpcr output (Biorad) ?

--Hi, is there a formula to compute the p-values from the ddPCR output ? I have an excel file outputed from Biorad QX600, i have a few columns with the CNV, PoissonCNVMax, PoissonCNVMax,...

28 November 2024 6,671 0 View

Best way to represent digital PCR relative expression ?

-Hi, is there some recommendations to represent digital PCR gene expression in barplot graph: Do i need to use ratio or concentration (copies/ul) ? thank you --

23 July 2024 2,146 1 View

DdPCR analysis: biological replicates ?

--Hi, such as RTqPCR to analyse gene expression, is it necessary to have biological replicates in a ddPCR experiment ? thank yu --

22 May 2024 8,226 6 View

What is the significance difference between a p-value of 0.01 and a p-value of 0.012 ?

--Hi, p-value = 0.01 and p-value = 0.012 is it risky to say that it is the same thing in terms of probability ? p=0.01 --> * * p=0.012 (p>0.01) --> * Thank you --

10 November 2022 7,479 3 View

Is it necessary to add error bar on control condition on qPCR histogram that represent fold change ?

--Hi, a reviewer ask me to add error bar on my qPCR control condition. But this is what i think: Noteably, the error bar for the control group has a different statistical meaning as the error...

07 October 2019 7,464 3 View

How to compute probability of high polymorphism region ?

I am working on a research project concerning the study of polymorphic regions of the genome. For that I use the DGV database. The question is the following: How to compute probability of high...

01 January 1970 4,951 0 View