Differentiation of the HL-60 cells to neutrophil-like cells with DMSO and ATRA?

The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI +...

06 June 2014 1,852 19 View