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Has anyone ever used multiple custom primers (read 1 primer, read 2 primer, and index primer) for amplicon sequencing? We would like to try to combine 16S custom barcoded amplicon primers with...
12 September 2018 8,916 1 View
Hi All, I am running PCR amplification with fungal primers ITS1 and ITS2. After running the PCR I am getting some samples that have an extra band. My samples are from human dental plaque, and I...
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