Lauren McNicholas

4 Questions 2 Answers 0 Followers

Questions related from Lauren McNicholas

How can I get LTP in slices without using Gabazine?

Hi all I am having trouble getting LTP in hippocampal slices (CA3-CA1) from 9-16-week-old WT mice. I can get LTP fine in aCSF containing 1uM gabazine (I cut CA3 to prevent epileptic activity)...

10 December 2021 2,568 2 View

Mouse hippocampal field recordings: why is the baseline unstable?

We are recording field EPSPs in CA3-CA1 synapses from 2-3-month old WT mice. We can get good EPSPs (size trace in blue) but during the baseline the EPSP gets small (yellow trace) and then large...

20 July 2021 4,181 2 View

Why has the pH of my aCSF changed (electrophysiology)?

Hi all, I make my aCSF using the following: 126 mM NaCl, 2 mM CaCl2, 10 mM glucose, 2 mM MgSO4.7H2O, 3 mM KCl, 1.25 mM NaH2PO4.2H2O and 26.4 mM NaHCO3 bubbled with carbogen gas (95% O2, 5% CO2)....

13 May 2021 6,854 1 View

How can I get reproducible LTP in WT slices?

Hi everyone I am performing field recordings to measure LTP in CA3-CA1 hippocampal slices from 9-week-old WT mice. This has been working well but recently I am having trouble getting...

25 February 2021 6,237 3 View