Lauren Farwell

3 Questions 1 Answers 0 Followers

Questions related from Lauren Farwell

How to calculate how much primer to add from pmols?

Hello all, I am quite new to performing qPCRs and am used to adding the primers in as a uM concentration. A paper I am following states the following in regards to a qPCR reaction mix: "For the...

22 February 2023 7,302 3 View

How to determine species I.D. of fungal isolates using a DNA barcoding region and BLAST?

Hello everyone, I have some questions in regards to using BLAST and sequencing data to give a species I.D. for fungal isolates. We have created pure isolates of fungal colonies and then...

06 January 2022 7,268 4 View

Why use a sigmoidal curve to calculate a threshold?

I have a response threshold that I have calculated using a sigmoidal curve fitted to my data set. What I want to understand is why this is a good method for finding a threshold over maybe setting...

02 January 2019 9,575 2 View