Laura Siracusa

2 Questions 1 Answers 0 Followers

Questions related from Laura Siracusa

DMEM/F12 causing high background in my commercial sandwich ELISA, any solutions?

I am growing adherent primary epithelial cells in supplemented DMEM/F12 and testing the supernatant for inflammatory cytokines. I purchased a commercial sandwich ELISA to test for analyte....

04 April 2019 7,778 3 View

Cell culture media (RPMI) ruining IL 1b sandwich ELISA?

I'm trying to measure the concentration of IL 1b in tracheal epithelial cells. Cells are grown in RPMI media. However, I have run the experiment several times and the amount of background is...

04 April 2019 2,168 2 View