Laura-Nadine Schuhmacher

2 Questions 7 Answers 0 Followers

Questions related from Laura-Nadine Schuhmacher

Does pIRES2 express more EGFP than the mRNA before the IRES?

Hi, I've subcloned all of my mRNAs into pIRES2 (CMV:mRNA-IRES-EGFP) for expression in cell culture (CHO and TSA cells) and electrophysiology. I have been getting a lot of bright green cells with...

04 April 2015 4,778 9 View

Why are my adult mouse astrocytes are not attaching?

Hi, I'm trying to make an adult (!) primary astrocyte culture from mouse cortex. I am doing this with a very experienced person who has done this before in a different lab. I am using her reagents...

04 April 2015 1,599 10 View