Laura Macrì Pellizzeri

3 Questions 5 Answers 0 Followers

Questions related from Laura Macrì Pellizzeri

Optimal conditions of Puromycin selection for immortalized human bone marrow MSCs ?

Dear ALL, i was wondering if somebody with experience can recommend the optimal conditions (in terms of concentration and time of incubation) of Puromycin selection for immortalized human bone...

06 June 2017 2,586 2 View

Transient transfection for luciferase assay ?

Hi,does anybody know how the expression of luciferase lasts upon transient transfection? I need to check the differentiation of MSCs after 3 weeks in culture, will it keep all this time? Thanks...

02 February 2016 5,619 3 View

Is the Oxytetracycline suitable to mark in vitro MSCs mineralization?

Hi, I am looking for a technique to label the MSCs mineralization foci in vitro. As I use a Ca/P-containing substrate, I can't use Alizarin Red o van Kossa staining... I have found that OTC has...

02 February 2016 8,280 4 View