Laura García-Pupo

3 Questions 7 Answers 0 Followers

Questions related from Laura García-Pupo

Which is the maximum tolerable value of Gibbs Free Energy for DNA template self dimer formation in qPCR?

Since the presence of secondary structures in primers and also DNA template has detrimental effects in PCR reactions, I was using the web tool...

07 July 2013 7,266 3 View

Prediction softwares for DNA secondary structures?

I´m designing some new primers for SYBR Green Real-time PCR, and I would like to be sure that there is a low probability of secondary structures formation in my primers and also in the template....

06 June 2013 4,186 6 View

Determining amplification efficiencies in qPCR?

For qPCR experiments I have several target genes and I need to know the amplification efficiencies with the selected primers. So I am taking the risk with perhaps a naïve question. In order to...

06 June 2013 3,302 8 View