Laura España Serrano

6 Questions 9 Answers 0 Followers

Questions related from Laura España Serrano

Hi, I am looking for a database/website where I can find the targets of antisense RNAs

I have a list of antisense RNAs and I would like to find their corresponding gene targets. I know you can find one by one, for example in ensembl website, but it is tedious and slow. Does anyone...

07 July 2017 6,167 5 View

Is there a way to modify a protein kinase assay by not drying the acrylamide gel?

I was used to follow the basic protocol for protein kinase assays incubating the sample with radioactive ATP and the purified kinase protein. Later I run the samples in an acrylamide gene and I...

08 August 2013 5,246 7 View

Consensus motif using MEME program. Is it real or is it only a statistically significant repetition?

I did a Chip Seq analysis and I used a MEME program to analyze the consensus motif for the transcription factor binding site. I obtained a long sequence (24 nucleotides). I did a search of this...

03 March 2013 5,734 8 View

How can I be sure if a TF predicted Consensus motif is real or only a frequent repeated sequence?

I used MEME program to analyze the consensus motif of the transcription factor binding site for a Chip Seq analysis. I did a search of this sequence (15 nucleotides) and I found that it is one of...

02 February 2013 5,154 0 View

Bioinformatic analysis of Chip-seq assay: do I need an Input sample to analyze the seq data? If so, how do I use it?

I did a triplicate of Chip assays, and I sent to sequence these 3 samples and one Input because I read I'll need it for the future bioinformatic analysis of the data. I don't have two different...

01 January 2013 5,566 10 View

What is the minimum number of reads needed to start the data analysis in a Chip-Seq assay?

I obtain around 10 million reads for each sample in my Chip-Seq assay . But after the Bioinformatics clean the sample, map and remove the duplicates we only have around 10% of the reads that we...

01 January 2013 3,937 3 View