Which is the best protocol for caspase-3 activity detection in vitro?

I'm trying to determine caspase-3 activity of cultured cells after treatment using the caspase-3 substrate (Ac-DMQD-AMC (Acetyl-Asp-Met-Gln-Asp- amino-4-methylcoumarin), fluorogenic), but the...

11 November 2014 447 7 View