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I have to evaluate and accurately quantify the effect of missense mutations on binding between two known interacting proteins. To this end, is it better measure SEAP protein using the Matchmaker...
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I subjected to SDS-PAGE and WB analysis the whole cell extract of E.coli cells to evealuate if a recombinant protein was expressed. Is it necessary to normalize? how? is there a...
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I have to do a Biotinylated RNA pull-down assay to verify the ability of two RNA binding proteins to bind RNA of interest. I’m working on designing the oligos. I wonder it is better to add biotin...
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