Laura Siracusa

2 Questions 1 Answers 0 Followers

Questions related from Laura Siracusa

Cell culture media (RPMI) ruining IL 1b sandwich ELISA?

I'm trying to measure the concentration of IL 1b in tracheal epithelial cells. Cells are grown in RPMI media. However, I have run the experiment several times and the amount of background is...

14 April 2019 1,988 2 View

DMEM/F12 causing high background in my commercial sandwich ELISA, any solutions?

I am growing adherent primary epithelial cells in supplemented DMEM/F12 and testing the supernatant for inflammatory cytokines. I purchased a commercial sandwich ELISA to test for analyte....

03 April 2019 1,808 3 View