Laura Murphy

3 Questions 3 Answers 0 Followers

Questions related from Laura Murphy

Yeast secreted protein all going into flock/foam during culturing? Help?

Hello, I have been attempting to express a protein in Pichia pastoris GS115 for some time now. I have just discovered via wesetrn blot that all of my protein is being precipitated/forms into this...

10 April 2024 9,408 6 View

Pink Pichia pastoris colonies after electroporation. Any Suggestions?

Hi all, once again electroporation of Pichia proves troublesome. I am electroporating GS115 strain with a pPIC9K plasmid. Following three days of growth, both the MD and YPD plates contained pink...

13 February 2023 3,116 3 View

Pichia Pastoris GS115 Growth Morphology after Electroporation?

Hi all, First time working with yeast and I have some questions. Following electroporation (1800V) of my linearized pPIC9K plasmid into GS115 I grew them up on MD agar as per the Invitrogen...

14 February 2022 6,869 2 View