Laura Loera-Lopez

3 Questions 3 Answers 0 Followers

Questions related from Laura Loera-Lopez

How to prep microwaved-fixed mouse brains for sectioning and fluorescent immunohistochemistry?

Our lab has recently gotten access to the Muromachi Microwave Fixation system which kills and stops all enzymatic activity within 1 second! VERY neat equipment, however, I am having difficulty...

24 February 2025 1,430 3 View

Immunohistochemistry staining coming out poorly following a bad perfusion. What is the cause?

I am staining mouse brain tissue sections using an anti-GFAP primary antibody (for astrocytes). The results are coming out pretty weird. Some sections have decent staining, and some others have...

24 April 2024 4,213 1 View

I forgot to add DAPI during immunohistochemistry but have not yet coverslipped! How to move forward?

I am currently performing immunohistochemistry on mouse brain sections with GFAP (goat) and Iba1 (rabbit) primary antibodies to visualize astrocytes and microglia. Today was day two of my IHC....

13 September 2023 210 3 View