Laura Glendinning

3 Questions 5 Answers 0 Followers

Questions related from Laura Glendinning

Why are my 260/280 values high after RNase tretament?

Hi, Having an odd experience with some DNA samples extracted from chicken feces. The DNA was first extracted from my samples using the Powersoil kit. I then treated these samples with a 1/10...

30 March 2018 6,522 4 View

How should we take into account negative controls in lung microbiota 16S sequencing?

Hi, I have been pondering this question for a while and was wondering what people's thoughts were? There seem to be multiple different methods of dealing with OTUs found in extraction kit and PCR...

24 November 2015 7,838 4 View

Diluted mock bacterial community contains contamination but not from reagents. Anyone have any suggestions for explanation?

I have just sequenced two different dilutions of the mock community HM-782D. For one of these mocks I followed the manufacturers instructions and didn't dilute my sample. For the other I followed...

21 January 2015 3,120 13 View