Laura García-Pupo

2 Questions 7 Answers 0 Followers

Questions related from Laura García-Pupo

Is it correct to use a region of interest from de genome for X coverage calculation in RNASeq experiments, instead of the whole genome size?

Hello everyone, Traditionally in sequencing, X coverage is determined as the product of the number of sequenced reads and their length, divided by the genome size. Regarding RNASeq experiments and...

15 September 2017 8,861 2 View

Which is the maximum tolerable value of Gibbs Free Energy for DNA template self dimer formation in qPCR?

Since the presence of secondary structures in primers and also DNA template has detrimental effects in PCR reactions, I was using the web tool...

03 July 2013 7,921 4 View