Laura Brion

3 Questions 8 Answers 0 Followers

Questions related from Laura Brion

Does polybrene solution degrades upon freeze and thaw cycles?

I have been infecting primary fibroblast with high efficiency using lentivirus + polybrene for a couple of times but lately I have experienced zero infection efficiency and I' suspecting the...

15 October 2016 9,790 3 View

Can anyone advise me on the best way to assess MMP1 activity? Also regarding casein zymography, does anyone have experience with that?

I would like to assess MMP1 activity the cheapest way

22 August 2013 8,852 1 View

Trouble with transfection of primary cell cultures of lung fibroblasts

I am working with human lung fibroblasts from LONZA but common lipofection protocols (lipofectamine and santa cruz transfection reagent) don't work. Has anyone tried to transfect this or similar...

25 July 2012 9,212 14 View