Can I stain cells disassociated from frozen tissue (not fixed) with fluorochromes for nucleic acids (i.e. DAPI, IP, SYBR Green, etc)?

I am working with disassociated cells taken from frozen tissue (-20 C) and need to stain their nucleus, but it is not working. I am unable to preserve fresh tissue in PFA or GLA before freezing it...

06 June 2019 9,323 2 View