Larissa Langhi

2 Questions 2 Answers 0 Followers

Questions related from Larissa Langhi

Is single-cell qPCR absolute or relative?

I've found in the literature both kinds of quantifications. The "absolute papers" say that absolute is better because there are many fluctuations among single-cell genes, and the "relative papers"...

14 March 2014 4,883 4 View

Can someone do flow cytometry with cells from fixed and frozen organs?

I have lymphoid organs from mice perfunded with paraformaldehyde. The organs were cryopreserved and frozen. Could I somehow "smoothen" the fixative bonds and macerate the organ with potter (or so)...

18 April 2013 3,864 7 View