Laramie Pence

3 Questions 3 Answers 0 Followers

Questions related from Laramie Pence

Anyone have experience using HRMA (High Resolution Melting Analysis) to identify deletions in amplicons larger than 500bp?

I'm trying to develop a genotyping assay to identify heterozygous vs. wild type mice, the mutants have a 1kb deletion in my gene of interest. I've tested several primers and have a couple sets...

05 April 2018 2,857 3 View

How to troubleshoot inconsistent genotyping results from mouse tails?

I am genotyping knockout mice with a pcr protocol that has worked very well up until this month. I use fresh mouse tails, toes, or ear snips. I lyse over night and use phenol/chloroform...

07 December 2017 7,247 3 View

Suggestions for a reliable Oct4 antibody for mouse blastocysts?

I'm trying to stain mouse blastocysts with antibodies for Cdx2 and Oct4, I've had great success with my Cdx2 antibody however the Oct4 staining is always hazy and non-specific. I'm using an...

20 September 2016 8,685 3 View