Why do my unstimulated BMDMs show lower absorbance in the MTT assay?

When I perform the MTT assay on BMDMs, the unstimulated cells show lower absorbance values, around 0.2–0.3 at 570 nm. What could be the reason for this? The assay was performed in a 96-well plate...

30 July 2025 3,302 1 View