Laqiwe Vier

2 Questions 1 Answers 0 Followers

Questions related from Laqiwe Vier

How should I deal with a beautiful crystal with a bad diffraction?

I'm working on a membrane protein. For now I have gotten many difference type crystal. I find the most beautiful crystal has the most bad diffraction (< 20A）while others (6~15A). But I don't...

12 December 2015 9,074 5 View

Why can't I purify my Pichia pastoris expressing membrane protein using CHAPS?

I have expressed a membrane protein in Pichia pastoris and can purify it with DDM or TritonX-100. However when I use 2%CHAPS(30-40mM,with CMC 8mM) to dissolve membrane fragment I failed. But I...

07 July 2015 8,632 3 View