How can I accelareate dehydration of mouse lung with 10% sucrose for frozen section?

After perfusion of 4% PFA, I put mouse lung in 4% PFA overnight in 4 °C . Then I remove mouse lung to 10% sucrose (PBS diluted) in 4 °C. 24h later, the lung is still floating. Clearly, there are...

10 August 2020 5,127 1 View