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The ultrafiltration/diafiltration is the last step for protein purification. During the ultrafiltration/diafiltration of protein solution, i found TWEEN 80 was adsorbed by membrane. I want to add...
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CHo cell was used to produce recombinant protein. In the shake flask the cell viability is very good. But in the 5L bioreactor the CHo cell viability will decrease quickly. Maybe the pH control...
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