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Questions related from Lan Chen
For the past several months, I've been having trouble in getting contamination from thawed Sf9 cells. For the cells that's frozen in 2014 by other people, I have no problem thawing and growing...
07 July 2017 2,828 3 View
Recently I've been purifying a 85kD protein from Rosetta. The yield is pretty good and the 260/280 is around 0.7-0.8. However, when I run this sample on FPLC superose 6, it showed very huge...
03 March 2017 2,264 6 View
I've been encountering a really weird problem with cloning. With the same restriction sites, same vector, same procedures, the PCR fragments that's 400bp and 900bp worked fine; however, the 1.1kb...
02 February 2017 3,653 11 View
I tried to purify a beta propeller domain fused to MBP protein from BL21. I got a quite decent amount of protein, however, it doesn't run at the expected size on SDS-PAGE gel. The expected size is...
02 February 2017 496 9 View
