Lan Chen

4 Questions 6 Answers 0 Followers

Questions related from Lan Chen

Can soluble protein aggregation affect pull down assay?

I recently purified two GST tag fragments that are below 100 amino acids and did pull down assays. However, neither of them interacted with the other purified protein. We know that the two...

03 May 2017 419 4 View

Cloning doesn't work for PCR fragment larger than 1kb?

I've been encountering a really weird problem with cloning. With the same restriction sites, same vector, same procedures, the PCR fragments that's 400bp and 900bp worked fine; however, the 1.1kb...

14 February 2017 4,179 10 View

Purified protein doesn't run at expected size on the SDS-PAGE gel?

I tried to purify a beta propeller domain fused to MBP protein from BL21. I got a quite decent amount of protein, however, it doesn't run at the expected size on SDS-PAGE gel. The expected size is...

14 February 2017 6,167 10 View

Baculovirus P1 stock and P2 stock both have white fiber-like stuff floating?

I've been using the baculovirus expression system for a year, but recent three months, I've seen the P1 and P2 virus grow white stuff inside. They are good within the first week and the protein...

14 October 2016 4,401 6 View