Lalitha Gavya S

2 Questions 3 Answers 0 Followers

Questions related from Lalitha Gavya S

What are the possible reasons for TALON beads precipitating during protein binding?

Hi, I am trying to purify a his-tagged recombinant protein using TALON affinity resin. During the binding step the beads get precipitated. I have tried using 50 mM Tris-Cl with 300 mM NaCl at...

02 February 2019 2,003 8 View

Hi, has someone used Strep tag for protein purification?

I am trying to purify a membrane protein and a transcription factor. We are planning to use Step tag for purification. Can someone shed light on this method? Is it better than traditional GST and...

10 October 2018 5,626 0 View