Microglia staining on frozen human brain biopsy tissue?

I tried Iba-1 (WAKO) to stain microglia from frozen human brain biopsy tissue but it didnt work. Frozen brain biopsy tissue (1-2 mm) was fixed in 4% PFA for 2 hrs, sucrose impregnated, stored in...

12 December 2012 9,828 8 View