Lakshitha Kaushal Dayananda

3 Questions 3 Answers 0 Followers

Questions related from Lakshitha Kaushal Dayananda

Is it obvious thing to see plasmid degradation within a couple of weeks?

Hi y'all, I ordered a plasmid - pET21a+ (size with the insert is around 8.7 kb) very recently and reconstituted it with TE buffer (a commercially purchased one) and stored at -20 C until I use....

24 July 2025 2,051 5 View

Hi all, I want to know which kinds of bacterial plasmids are suitable for expressing human NAT10?

I wish to express human NAT10 enzyme in a bacterial system. Are there any codon optimized commercially available bacterial vector plasmids that facilitate this? If this is not feasible then what...

26 May 2025 7,611 1 View

Human NAT10 expression and SDS PAGE

Hi all, Recently, I expressed human NAT10 in Rosetta(DE3) at a 2 uM IPTG level (final concentration). I used 50 mL culture for this as I did for the first time (thinking to start with minimum...

01 January 1970 1,375 1 View