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Questions related from Lael Werner
Anybody has experience with a kit for library preparation from DNA. For T and B immune repertoire , for illumina sequencing. BUT samples are from FFPE!! I use Lymphotrak and it is great with...
07 July 2019 4,939 1 View
Hello. Need help with (eBioscience) FoxP3 kit. I heard the external antibodies (CD4 and CD25) can be added, AFTER the fix/perm, after overnight incubation at 4C, CONCURRENTLY with the Foxp3...
01 January 2017 2,057 5 View
How much IL-10 can be expected to secreted from 1million (freshly isolated and magnetically seperated, human) CD4+ cells, stimulated for 24 and/or 48 hours 1ug/ml plated CD3+ 2ug/ml soluble...
12 December 2016 3,958 2 View
I have been activating T cells with anti-CD3 (either soluble or plate bound) for many years. Currently, I am establishing a protocol whereby at the time of coating (or subsequently) I plate bind...
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