La So

3 Questions 4 Answers 0 Followers

Questions related from La So

Riboprobe for invitro transcription _ one band on the gel?

Hi I did the riboprobe with Sp6 polymerase after BamHI digestion of plasmid DNA . After running the gel, I got only one band (18s?). is my RNA degraded ? I need to Insitubhybridisation. Is my DNA...

17 September 2024 8,793 0 View

T7 or SP6 orientation?

I am planning to do Insitu-hybridisation. I have done the cloning finally . I cloned the PCR product in pGEMT easy vector . after doing the miniprep. I did the restriction digestion with...

19 March 2024 518 3 View

PGEMT-easy vector cloning ...very difficult ?

Hello everyone, I am trying to clone the gene of interest for the mRNA hybridization ( in situ) after cloning, and miniprepre and restriction digestion, . I don't get the desired product on the...

05 March 2024 9,747 4 View