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Questions related from Kyutae Park
protein sample should be intact in -70c as far as I know. but when I stored them 1 week after first western blot, I can't even detect b-actin signal in the second experiment. I used cold RIPA...
27 January 2025 270 2 View
our centrifuge is broken, so I had to centrifuge my cell lysate in RIPA buffer with protease inhibitor for western blot at room temperature not 4*C for 20min. is that OK..?
31 August 2024 3,095 0 View
