Is any special factor required to clone I-SceI gene from S. cerevisiae?

I am currently cloning codon-optimized I-SceI gene (from S. cerevisiae) into pET vector using E. coli DH5a, but I am keep failing cloning. Even if I assume a leaky expression of I-SceI cloned into...

29 November 2017 6,247 3 View