Kurt M. Yakimovich

2 Questions 4 Answers 0 Followers

Questions related from Kurt M. Yakimovich

Why do gDNA concentrations need to be standardized going into the first PCR during amplicon seq library prep for a MiSeq?

I am looking for a more technical answer, I see the standard illumina protocols suggest it, but can't find a clear explanation why. I assume it has something to do with standardizing reaction...

04 April 2019 2,851 8 View

Looking for insights into why two sequences don't cluster closely together on phylogenetic tree despite aligning with 99% identity and 97% querycover?

I used RDP classifier in my analysis pipeline of some mcrA amplicon data which assigned my OTU 1 the identity of a specific clone (MCR12). However, if I include that clone's mcrA sequence in my...

01 January 2017 9,996 6 View