Kunthavai Balasubramanian

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Questions related from Kunthavai Balasubramanian

How to improve the purity of CD34+ cells while isolating from Cord Blood using MACS column? Can we use another column to elute the eluted CD34+ again?

I am trying to isolate CD34+ cells from cord blood and I have been getting 75% purity each time.So I am wondering have anyone tried using another column to elute them again. Will that help in...

06 June 2016 7,976 1 View

I have fixed human cells using 4% PFA on a cover slip and left them in PBS at 4 degree. How long can I leave my fixed cells in PBS before IF?

If I want to store them for longer period do I have to do antibody retrieval before staining? Thank you.

03 March 2016 5,637 3 View