Kumar Avishek

3 Questions 6 Answers 0 Followers

Questions related from Kumar Avishek

Is it necessary to do immunofluorescence assay (IFA) immediately after fixation of cells?

I am planning to do IFA to see the intracellular localization of a protein in Leishmania parasite. Is it necessary to do IFA just after fixation of cells on slides or I can store these slides for...

08 August 2014 1,027 11 View

What is the maximum number of freeze thaw cycle that a protein can tolerate?

I am planning to do western blot to see the expression of my protein of interest in total cell lysate. Its always recommended not to freeze thaw a protein more than 2 times. But as a beginner I...

08 August 2014 8,331 5 View

Intacellular staining for T-cell and macrophage cytokines?

I am looking for the cytokines in cultured PBMCs that are stimulated in response to antigen. Mainly I am interested in cytokines secreted by T cells (th1/th2) and macrophages. I have already...

06 June 2013 3,725 2 View