Abhishek Kumar

2 Questions 3 Answers 0 Followers

Questions related from Abhishek Kumar

What could be the resean for missing 200 bp DNA from a clone during infusion cloning?

Hi Everyone, I am trying to sub-clone TCF7 gene (transcript variant 1) into pCDH-CMV-MCS-EF1 Puro vector from some other commercially available vector by InFusion cloning(recombination). I got...

06 November 2020 6,436 2 View

What are the methods to know the retrovirus positive transduced cells after infecting with retrovirus and antibiotic selection?

Hi I generated retrovirus by transfecting the plasmid with my gene of interest in packaging cell line and checked my gene in generated retrovirus by RT-PCR. When I infecting HEK 293 cell line with...

18 February 2019 9,596 3 View