Kulani Mhlongo

4 Questions 2 Answers 0 Followers

Questions related from Kulani Mhlongo

What is the best way of purifying secondary metabolites from a butanol extract?

I want to identify the active compounds from the fraction.

17 January 2018 9,249 3 View

Is there any other media one can use instead of MS agar during conjugation of Streptomyces Lividans? It seems not to be working for some reason?

I tried using Normal LB and YPD still no success. I am conjugation using E.coli S17.1.

26 July 2016 8,473 8 View

Is there a way to make Streptomyces Livands sporulate and quick?

Currently inducing sporalution with addition of Proline and Cacl2 into media (YEP).

26 July 2016 2,778 1 View

Does anyone know why when I extract Natural products from my fermentation broth using anion beads I get activity towards gram positive bacteria?

I specifically get activity against B. cerues and S. Epidermis after doing an methanol exaction. But when I use Cation beads I see activity for both Gram positive and negative.

10 March 2015 5,170 4 View