Are there decapping buffers for DCP2 without Tris and/or magnesium chloride? Can I replace Tris in my buffer?

I found out that likely factor, causing degradation of my RNAs during decapping with hDCP2, is non-enzymatic reaction caused by Tris-HCl and Mg2+. All alternative buffers I found contain both in...

06 April 2022 6,724 0 View