Krupal Jethava

4 Questions 13 Answers 0 Followers

Questions related from Krupal Jethava

How to purify amyloid-beta(1-42) conjugated with small molecule?

I am conjugating Amyloid-beta (Abeta) peptide (MW ~4500) with a small molecule (starting with monomeric abeta). I tried purifying the product with protein concentrator with 3000 molecular weight...

15 August 2019 6,646 5 View

Can anyone suggest about how to purify pure protein (Mol Wt: 5K) from the crude having small molecule (Mol Wt: 500)?

I would like to use some method that can remove small molecule impurities from peptide (which has around 4K-5K molecular wt) so that I can get peptide without contaminant. Can anyone suggest...

22 March 2018 4,956 4 View

How can I calculate %yield from 1H-NMR analysis using some reference standard?

I would like to have some information or step by step process to calculate %yield of product from taking 1H-NMR of reaction mixture. Can anyone help in this regards?

10 April 2015 9,078 30 View

What is the coupling constant for CF3 carbon in 13C-NMR?

If we have to report a CF3 functional group containing a compound, then what is the range of the J value (coupling constant) in a 13C-NMR for CF3 carbon and carbon 'attached to' CF3 in simple...

23 June 2014 2,545 5 View