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Questions related from Kritan Gautam
I need help in creating stable cell lines.I have a gfp tagged protein in pcDNA 3.1.It has zeocin resistance.I want my transfected Hek293T cells produce this gfp tagged protein stably.What would be...
03 March 2012 1,856 10 View
does anybody know about the interaction between these Tra O and Tra N protein?any articles ?recomendations?
03 March 2012 7,689 0 View
I am trying to purify my protein from the cell culture supernatant. I transfected my cells with my construct (which has a double his tag) to produce my protein in the supernatant. I collected the...
03 March 2012 6,965 6 View
I am having problem in expressing my protein which has a m.cherry tag..does anybody here have ever faced this problem ?
02 February 2012 8,920 4 View
I have a cytokine displayed on the bacterial cell surface and I need to check the binding of this cytokine with gfp tagged receptor ectodomain in Flow cytometry..the problem i am having is to gate...
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