Qiagen micro RNA primers generate PCR product in negative control, but not in positive control. How could this be?

I used Qiagen miRNEasy micro kit, miScript RT II kit and miScript SYBR greenPCR kit with Qiagen custon primers assays for mir-499 and mir-208. No-matrix PCR showed one-peak melting curve and the...

10 October 2019 9,042 3 View