Kristina Klein

3 Questions 7 Answers 0 Followers

Questions related from Kristina Klein

Does somebody have a recipe for non-radioactive EMSA blocking and wash buffer?

Hi, I want to use an Affymetrix kit for EMSA (AY1000) with biotin-labeled probes, but the blocking and wash buffers are already empty. Do you know the components of the buffers? I found so many...

11 November 2014 6,328 9 View

Do I have to strip my membrane when I want to detect the same protein but with a primary antibody from another company?

Hey everybody! I have no signal for my protein (IkappaBalpha), but the control GAPDH is there. So I want to try an antibody from another company now. Is it necessary to stripp before incubating...

08 August 2014 2,339 7 View

How can I label RNA in bacteria?

Is there a possibility to label RNA (siRNA), with e.g. a fluorescent marker, in bacteria (Helicobacter pylori) to see if it secretes it into its target eukaryotic cells? I only know a kit for...

08 August 2014 7,471 3 View