Multi-plasmid transfection 293T; Losing expression of a protein. How to fix?

I need to transfect (Lipofectamine 2000) 4 expression plasmids (totaling 10.1 microG all containing CMV or CMV minimal promoters) into 293T cells (>90% confluent at time of transfection in 10cm...

05 December 2016 9,163 3 View